High-density oligonucleotide arrays
WebAlthough oligonucleotide probes are often used in "spotted" microarrays, the term "oligonucleotide array" most often refers to a specific technique of manufacturing. … WebHigh-density oligonucleotide-based array. (A) Microarray fabrication by photolithographic synthesis, which involves repeated cycles of targeted deprotection, coupling, and protection of the coupled bases. (B) Microarray using fluorescent-dye-labeled targets and competitive hybridization of the two probes on the same array slide.
High-density oligonucleotide arrays
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Web18 de mai. de 2007 · In spotted arrays, complementary DNA or long oligonucleotide probes are printed onto the substrate. The two messenger RNA samples of interest are reverse transcribed into complementary DNA, labelled with a different fluorescent dye (green cyanine 3 or red cyanine 5) and mixed together, before being washed over the slide and …
Web23 de dez. de 2002 · We used high-density oligonucleotide microarrays to examine the short- and long-term actions of estradiol (E 2) on the transcriptomes from the medial basal hypothalamus and other brain regions of E 2-treated (10 μg) adult female mice. Our results have revealed several unanticipated gene regulations. WebHigh-density oligonucleotide expression arrays are a widely used microarray platform. Affymetrix GeneChip arrays dominate this market. An important distinction between the GeneChip and other technologies is that on GeneChips, multiple short probes are used to measure gene expression levels.
WebThis work presents a low-cost method to produce compact arrays using microporous materials and reagent jetting, particularly useful for producing identical arrays from pre … Web1 de nov. de 1996 · High-density arrays of oligonucleotide probes are proving to be powerful new tools for large-scale DNA and RNA sequence analysis. A method for constructing these arrays, using light-directed DNA synthesis with photoactivatable monomers, can currently achieve densities on the order of 10 6 sequences/cm 2.One of …
WebHepatic NK cells are more cytotoxic than blood NK cells against tumor cells. To understand the basis of this difference in cytotoxicity we analyzed RNA derived from freshly isolated …
Web1 de jan. de 2002 · The leading arrays in the category of high-density oligonucleotide arrays are manufactured by Affymetrix and utilize the combinatorial synthesis principle. 9 The arrays are designed by using a light-directed chemical synthesis process that creates a series of photolithographic masks to define chip exposure sites, followed by specific … fishing osrs f2pWeb1 de dez. de 1996 · Hacia, J., Brody, L., Chee, M. et al. Detection of heterozygous mutations in BRCA1 using high density oligonucleotide arrays and two–colour fluorescence analysis. Nat Genet 14 , 441–447 (1996 ... can cancer cause joint pain and swellingWebFigure 2: Gene expression monitoring with oligonucleotide arrays. a, A single 1.28×1.28 cm array containing probe sets for approximately 40,000 human genes and ESTs. This array contains features ... fishing osrs calculatorWebHigh-density DNA probe arrays provide a highly parallel approach to nucleic acid sequence analysis that is transforming gene-based biomedical research. … can cancer cause excessive sweatingWebAn approach in which sequence information is used directly to design high–density, two–dimensional arrays of synthetic oligonucleotides is developed, which have been … can cancer cause increased wbcWebMultiple microarray platforms exist, including printed double-stranded DNA and oligonucleotide arrays, in situ-synthesized arrays, high-density bead arrays, electronic microarrays, and suspension bead can cancer cause flu like symptomsWebWe explored the utility of high-density oligonucleotide arrays {DNA chips) for obtaining sequence information from homolo gous genes in closely related species. can cancer cause lower back pain